BLM anticorps (AA 1319-1335)

N° du produit ABIN303411

Détail du produit anti-BLM anticorps

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Épitope: AA 1319-1335

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BLM est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: Recognises Bloom's Syndrome Protein (BLM).

Purification: Protein G Chromatography.

Immunogène: Synthetic Peptide - KLH conjugated corresponding to Amino acids 1319 to 1335 of Human Bloom Syndrome protein (BLM).

Information d'application

Indications d'application: ELISA (1/0-1/1000). Immunohistochemistry on Paraffin Sections (10 μg/mL).

Restrictions: For Research Use only

Stockage

Buffer: Phosphate Buffered Saline PBS containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Détails sur BLM

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Autre désignation: BLM (BLM Produits)

Synonymes: anticorps BS, anticorps RECQ2, anticorps RECQL2, anticorps RECQL3, anticorps blm-A, anticorps xBLM, anticorps ORF1, anticorps Bloom syndrome RecQ like helicase, anticorps Bloom syndrome RecQ like helicase S homeolog, anticorps Bloom syndrome, RecQ like helicase, anticorps BLM, anticorps blm.S, anticorps Blm

Sujet: The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44 % amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions. The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process. Bloom syndrome cells show marked genomic instability, in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.Synonyms: Bloom syndrome protein, DNA helicase, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like type 2

ID gène: 641

NCBI Accession: NP_000048

UniProt: P54132

Pathways: Réparation de l'ADN