UCHL1 anticorps (C-Term)

N° du produit ABIN3042339

Cet anticorps anti-UCHL1 est un anticorps Lapin Polyclonal détectant UCHL1 dans WB, IHC, IF, FACS et ICC. Adapté pour Humain, Rat et Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour UCHL1 anticorps (C-Term) (ABIN3042339)

Antigène: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UCHL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-UCHL1 anticorps

Épitope: AA 120-153, C-Term

Fonction: Anti-PGP9.5/UCHL1 Antibody

Séquence: ETEKMSPEDR AKCFEKNEAI QAAHDAVAQE GQCR

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PGP9.5/UCHL1 Antibody (ABIN3042339). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-UCHL1 (ABIN3042339)

Han, Chen, Huang, Zhu: "Nude mice multi-drug resistance model of orthotopic transplantation of liver neoplasm and Tc-99m MIBI SPECT on p-glycoprotein." dans: World journal of gastroenterology, Vol. 11, Issue 22, pp. 3335-8, (2005) (PubMed).

Détails sur UCHL1

Antigène: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Autre désignation: UCHL1

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase isozyme L1,UCH-L1,3.4.19.12,6.-.-.-,Neuron cytoplasmic protein 9.5,PGP 9.5,PGP9.5,Ubiquitin thioesterase L1,UCHL1,

Tissue Specificity: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. .

Background: UCH-L1, also known as PGP9.5, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2 % of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.

Poids moléculaire: 25 kDa

ID gène: 7345

UniProt: P09936

Pathways: Feeding Behaviour