Menin anticorps (AA 301-615)

N° du produit ABIN3042359

L’anticorps Lapin Polyclonal anti-Menin a été validé pour WB. Il convient pour détecter Menin dans des échantillons de Humain et Souris.

Aperçu rapide pour Menin anticorps (AA 301-615) (ABIN3042359)

Antigène: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Menin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Menin anticorps

Épitope: AA 301-615

Fonction: Anti-Menin/MEN1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Menin/MEN1 Antibody Picoband® (ABIN3042359). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Menin recombinant protein (Position: P301-L615). Human Menin shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Menin, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Canaff, L., Vanbellinghen, J.-F., Kaji, H., Goltzman, D., Hendy, G. N. Impaired transforming growth factor-beta (TGF-beta) transcriptional activity and cell proliferation control of a menin in-frame deletion mutant associated with multiple endocrine neoplasia type 1 (MEN1). J. Biol. Chem. 287: 8584-8597, 2012. 2. Kaji, H., Canaff, L., Lebrun, J.-J., Goltzman, D., Hendy, G. N. Inactivation of menin, a Smad3-interacting protein, blocks transforming growth factor type beta signaling. Proc. Nat. Acad. Sci. 98: 3837-3842, 2001. 3. Zablewska, B., Bylund, L., Mandic, S. A., Fromaget, M., Gaudray, P., Weber, G. Transcription regulation of the multiple endocrine neoplasia type 1 gene in human and mouse. J. Clin. Endocr. Metab. 88: 3845-3851, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Menin

Antigène: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Autre désignation: MEN1

Sujet:

Synonyms: Menin,MEN1,SCG2,

Tissue Specificity: Ubiquitous.

Background: The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. What's more, MEN1 was essential to maintain MLL-associated myeloid transformation.

Poids moléculaire: 68 kDa

ID gène: 4221

UniProt: O00255

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity