GCLC anticorps (AA 437-637)

N° du produit ABIN3042420

Détail du produit anti-GCLC anticorps

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Épitope: AA 437-637

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: glutamate-cysteine ligase, catalytic subunit
Protein Name: Glutamate--cysteine ligase catalytic subunit

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for GCLC is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-GCLC (ABIN3042420)

Ni, Wang, Qiu, Pang, Song, Guo: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).

Jin, Li, Zhang, Wu, Feng, Zhang, Shen: "Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway." dans: Neural regeneration research, Vol. 11, Issue 7, pp. 1090-8, (2016) (PubMed).

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: GCLC (GCLC Produits)

Synonymes: anticorps GCLC, anticorps gclc, anticorps MGC146287, anticorps 2259, anticorps CG2259, anticorps DmGCLC, anticorps DmGCS, anticorps DmGCSh, anticorps Dmel\\CG2259, anticorps GCL, anticorps GCLc, anticorps GCSh, anticorps Gcs, anticorps Gcsh, anticorps DDBDRAFT_0186120, anticorps DDBDRAFT_0231403, anticorps DDB_0186120, anticorps DDB_0231403, anticorps GCS, anticorps GLCL, anticorps GLCLC, anticorps D9Wsu168e, anticorps GLCL-H, anticorps Ggcs-hs, anticorps Glclc, anticorps cb1049, anticorps fe36e11, anticorps wu:fe36e11, anticorps glutamate-cysteine ligase catalytic subunit, anticorps glutamate-cysteine ligase, catalytic subunit, anticorps Glutamate-cysteine ligase catalytic subunit, anticorps gamma glutamylcysteine synthetase, anticorps glutamate-cysteine ligase, catalytic subunit L homeolog, anticorps glutamate-cysteine ligase, catalytic subunit S homeolog, anticorps gamma-glutamylcysteine synthetase, anticorps GCLC, anticorps gclc, anticorps Gclc, anticorps LbGCS, anticorps gcsA, anticorps gclc.L, anticorps gclc.S, anticorps GSH1

Sujet: GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Synonyms: EC 6.3.2.2 antibody|Gamma ECS antibody|Gamma GCS antibody|Gamma glutamylcysteine synthetase antibody|Gamma-ECS antibody|Gamma-glutamylcysteine synthetase antibody|GCL antibody|GCLC antibody|GCLM antibody|GCS antibody|GCS Heavy Chain antibody|GLCL antibody|GLCLC antibody|GLCLR antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase (gamma glutamylcysteine synthetase) regulatory (30.8kD) antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase modifier subunit antibody|Glutamate cysteine ligase regulatory protein antibody|Glutamate cysteine ligase regulatory subunit antibody|Glutamate--cysteine ligase catalytic subunit antibody|GSC light chain antibody|GSH1_HUMAN antibody

ID gène: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis