ATG7 anticorps (C-Term)

N° du produit ABIN3042570

Cet anticorps anti-ATG7 est un anticorps Lapin Polyclonal détectant ATG7 dans WB et FACS. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour ATG7 anticorps (C-Term) (ABIN3042570)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-ATG7 anticorps

Épitope: AA 652-669, C-Term

Fonction: Anti-Apg7/ATG7 Antibody Picoband®

Séquence: KVLDQYEREG FNFLAKVF

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Apg7/ATG7 Antibody (ABIN3042570). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae)" 2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Note: Erratum: Science 341: 457 only, 2013. 3. Tanida, I., Tanida-Miyake, T., Ueno, T., Kominami, E. The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem. 276: 1701-1706, 2001.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-ATG7 (ABIN3042570)

Shen, Li, Cai, Liu, Wang, Gan, Li, Wang, Sun, Deng, Liu, Li: "Erbin exerts a protective effect against inflammatory bowel disease by suppressing autophagic cell death." dans: Oncotarget, Vol. 9, Issue 15, pp. 12035-12049, (2018) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." dans: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: ATG7

Sujet:

Synonyms: Ubiquitin-like modifier-activating enzyme ATG7,ATG12-activating enzyme E1 ATG7,Autophagy-related protein 7,APG7-like,hAGP7,Ubiquitin-activating enzyme E1-like protein,ATG7,APG7L,

Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Background: Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family.

Poids moléculaire: 78 kDa

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy