CYP27B1 anticorps (C-Term)

N° du produit ABIN3042787

Cet anticorps Lapin Polyclonal détecte spécifiquement CYP27B1 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CYP27B1 anticorps (C-Term) (ABIN3042787)

Antigène: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CYP27B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CYP27B1 anticorps

Épitope: AA 475-508, C-Term

Fonction: Anti-CYP27B1 Antibody

Séquence: HFEVQPEPGA APVRPKTRTV LVPERSINLQ FLDR

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CYP27B1 Antibody (ABIN3042787). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CYP27B1

Antigène: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Autre désignation: CYP27B1

Sujet:

Synonyms: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial,1.14.13.13,25-OHD-1 alpha-hydroxylase,25-hydroxyvitamin D (3) 1-alpha-hydroxylase,VD3 1A hydroxylase,Calcidiol 1-monooxygenase,Cytochrome P450 subfamily XXVIIB polypeptide 1,Cytochrome P450C1 alpha,Cytochrome P450VD1-alpha,Cytochrome p450 27B1,CYP27B1,CYP1ALPHA, CYP27B,

Tissue Specificity: Kidney.

Background: CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Sequence Similarities: Belongs to the cytochrome P450 family.

Poids moléculaire: 57 kDa

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D