TOLLIP anticorps (C-Term)

N° du produit ABIN3042954

Cet anticorps anti-TOLLIP est un anticorps Lapin Polyclonal détectant TOLLIP dans WB, IHC, IF, ICC et FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour TOLLIP anticorps (C-Term) (ABIN3042954)

Antigène: TOLLIP (Toll Interacting Protein (TOLLIP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOLLIP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-TOLLIP anticorps

Épitope: AA 224-243, C-Term

Fonction: Anti-Toll-interacting protein Tollip Antibody Picoband®

Séquence: NAQPRCSEED LKAIQDMFPN

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Toll-interacting protein Tollip Antibody (ABIN3042954). Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Tollip.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bulut, Y., Faure, E., Thomas, L., Equils, O., Arditi, M. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling. J. Immun. 167: 987-994, 2001. 2. Burns, K., Clatworthy, J., Martin, L., Martinon, F., Plumpton, C., Maschera, B., Lewis, A., Ray, K., Tschopp, J., Volpe, F. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nature Cell Biol. 2: 346-351, 2000. 3. Lo, Y.-L. S., Beckhouse, A. G., Boulus, S. L., Wells, C. A. Diversification of TOLLIP isoforms in mouse and man. Mammalian Genome 20: 305-314, 2009.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-TOLLIP (ABIN3042954)

Liu, Wang, Qian, Li: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." dans: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, (2014) (PubMed).

Détails sur TOLLIP

Antigène: TOLLIP (Toll Interacting Protein (TOLLIP))

Autre désignation: TOLLIP

Sujet:

Synonyms: Toll-interacting protein,TOLLIP,

Tissue Specificity: Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.

Background: TOLLIP (TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al. (2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al. (2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al. (2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.

Sequence Similarities: Belongs to the tollip family.

Poids moléculaire: 30 kDa

UniProt: Q9H0E2