ITGA4 anticorps (C-Term)

N° du produit ABIN3043255

Cet anticorps Lapin Polyclonal détecte spécifiquement ITGA4 dans FACS, WB, IHC et IHC (fro). Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour ITGA4 anticorps (C-Term) (ABIN3043255)

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ITGA4 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-ITGA4 anticorps

Épitope: AA 999-1028, C-Term

Fonction: Anti-Integrin alpha 4/ITGA4 Antibody Picoband®

Séquence: MWKAGFFKRQ YKSILQEENR RDSWSYINS

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Integrin alpha 4/ITGA4 Antibody Picoband® (ABIN3043255). Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4, different from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Jaspers, M., Zhang, Z., Marynen, P., Vekemans, S., Aly, M. S., Cuppens, H., Hillicker, C., Cassiman, J.-J.Localization of the genes encoding the alpha-2 and alpha-4 subunits of the human VLA-receptors to chromosome 5q23-31 and 2q31-32 respectively. (Abstract)Cytogenet. Cell Genet. 58: 1870 only, 1991. 2. Miller, D. H., Khan, O. A., Sheremata, W. A., Blumhardt, L. D., Rice, G. P. A., Libonati, M. A., Willmer-Hulme, A. J., Dalton, C. M., Miszkiel, K. A., O'Connor, P. W.A controlled trial of natalizumab for relapsing multiple sclerosis.New Eng. J. Med. 348: 15-23, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ITGA4

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Autre désignation: ITGA4

Sujet:

Synonyms: Integrin alpha-4,CD49 antigen-like family member D,Integrin alpha-IV,VLA-4 subunit alpha,CD49d,ITGA4,CD49D,

Tissue Specificity: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U-937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.

Background: ITGA4/Integrin alpha4 is an integrin alpha subunit. It makes up half of the α4β1 lymphocyte homing receptor. The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. ITGA4 is also mapped to 2q31-q32 by fluorescence in situ hybridization.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Poids moléculaire: 220 kDa

ID gène: 3676

UniProt: P13612

Pathways: Integrin Complex