PKM anticorps (N-Term)

N° du produit ABIN3043381

Détail du produit anti-PKM anticorps

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Épitope: AA 475-500, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.

Séquence: KDPVQEAWAE DVDLRVNFAM NVGKAR

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.
Gene Name: pyruvate kinase, muscle
Protein Name: Pyruvate kinase PKM

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PKM2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM (PKM Produits)

Synonymes: anticorps CTHBP, anticorps OIP3, anticorps PK3, anticorps PKM2, anticorps TCB, anticorps THBP1, anticorps AA414905, anticorps AL024370, anticorps AL024424, anticorps Pk-2, anticorps Pk-3, anticorps Pk3, anticorps Pkm2, anticorps PKM12, anticorps CG7070, anticorps Dmel\\CG7070, anticorps PYK, anticorps Pyk, anticorps cb1029, anticorps fj33f03, anticorps pkm2, anticorps pkm2a, anticorps wu:fj33f03, anticorps MGC53388, anticorps PKM, anticorps PK-S, anticorps pk3, anticorps pkm2b, anticorps zgc:92037, anticorps pyruvate kinase M1/2, anticorps pyruvate kinase, muscle, anticorps Pyruvate kinase, anticorps pyruvate kinase M1/2a, anticorps pyruvate kinase PKM, anticorps pyruvate kinase (PK), anticorps pyruvate kinase, anticorps pyruvate kinase M1/2 S homeolog, anticorps pyruvate kinase M1/2b, anticorps PKM, anticorps Pkm, anticorps PyK, anticorps pkma, anticorps pkm, anticorps LOC710798, anticorps pyk, anticorps pk, anticorps LOC100534543, anticorps pkm.S, anticorps pkmb

Sujet: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Synonyms: CTHBP antibody|Cytosolic thyroid hormone binding protein antibody|Cytosolic thyroid hormone-binding protein antibody|KPYM_HUMAN antibody|MGC3932 antibody|OIP 3 antibody|OIP-3 antibody|Oip3 antibody|OIP3 antibody|OPA interacting protein 3 antibody|Opa-interacting protein 3 antibody|p58 antibody|PK Muscle type antibody|PK, muscle type antibody|PK2 antibody|Pk3 antibody|PKM antibody|PKM2 antibody|PYKM antibody|Pyruvate kinase 2/3 antibody|Pyruvate kinase 3 antibody|Pyruvate kinase isozymes M1/M2 antibody|Pyruvate kinase muscle antibody|Pyruvate kinase muscle isozyme antibody|Pyruvate kinase, muscle 2 antibody|TCB antibody|THBP1 antibody|Thyroid hormone binding protein 1 antibody|Thyroid hormone binding protein cytosolic antibody|Thyroid hormone-binding protein 1 antibody|Tumor M2 PK antibody|Tumor M2-PK antibody

ID gène: 5315

UniProt: P14618

Pathways: L'effet Warburg