CD34 anticorps (AA 151-385)

N° du produit ABIN3043391

L’anticorps Lapin Polyclonal anti-CD34 a été validé pour WB, IHC, IF et ELISA. Il convient pour détecter CD34 dans des échantillons de Humain et Rat. Il y a 83+ publications disponibles.

Aperçu rapide pour CD34 anticorps (AA 151-385) (ABIN3043391)

Antigène: CD34

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-CD34 anticorps

Épitope: AA 151-385

Fonction: Anti-CD34 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CD34 Antibody Picoband® (ABIN3043391). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Satterthwaite, A. B., Burn, T. C., Le Beau, M. M., Tenen, D. G. Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. Genomics 12: 788-794, 1992. 2. Tenen, D. G., Satterthwaite, A. B., Borson, R., Simmons, D., Eddy, R. L., Shows, T. B. Chromosome 1 localization of the gene for CD34, a surface antigen of human stem cells. Cytogenet. Cell Genet. 53: 55-57, 1990. 3. Cheng, J., Baumhueter, S., Cacalano, G., Carver-Moore, K., Thibodeaux, H., Thomas, R., Broxmeyer, H. E., Cooper, S., Hague, N., Moore, M., Lasky, L. A. Hematopoietic defects in mice lacking the sialomucin CD34. Blood 87: 479-490, 1996.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-CD34 (ABIN3043391)

Zhang, Chen, Wang, Yang, Li, Wang, Liu, Ye: "Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase." dans: Oncotarget, Vol. 7, Issue 26, pp. 39302-39315, (2018) (PubMed).

Hu, Li, Zhang, Zheng, Wang, Zhang, Zhang, Gu, Ye, Guo, Yang, Wang: "Phosphoinositide 3-Kinase (PI3K) Subunit p110δ Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse." dans: Scientific reports, Vol. 6, pp. 28201, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Chu, Zhang: "Inhibition of angiogenesis by leflunomide via targeting the soluble ephrin-A1/EphA2 system in bladder cancer." dans: Scientific reports, Vol. 8, Issue 1, pp. 1539, (2018) (PubMed).

Zhang, Yuan, Wang, Shao, Liu, Firestone, Hong, Li, Xin, Li: "Experimental evidence of good efficacy and reduced toxicity with peptide-doxorubicin to treat gastric cancer." dans: Oncotarget, Vol. 9, Issue 2, pp. 1957-1968, (2018) (PubMed).

Shi, Lv, Nie, Lu, Liu, Tian, Li, Zhang, Ren, Zhang, Kang: "Human nail stem cells are retained but hypofunctional during aging." dans: Journal of molecular histology, Vol. 49, Issue 3, pp. 303-316, (2018) (PubMed).

Zhao, Feng, Sun, Qin, Zhang, Tan, Gao, Lau, Ma, Yang, Yu, Xu, Yi, Yi: "Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis." dans: Cell death & disease, Vol. 9, Issue 7, pp. 723, (2018) (PubMed).

Chen, Zhou, Yao, Dai, Zhou, Wang, Zhang: "Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway." dans: Journal of cellular and molecular medicine, Vol. 22, Issue 10, pp. 4760-4770, (2018) (PubMed).

Wan, Wu, Huang, Ge, Liu: "Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1α." dans: Oncology reports, Vol. 36, Issue 2, pp. 659-68, (2017) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." dans: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." dans: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, (2017) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." dans: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

Shi, Jiang, Wang, Cao, Liu, Xu, Wang, Sun, Niu: "Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device." dans: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 19, pp. 3058-65, (2016) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." dans: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." dans: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." dans: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Jiang, Li, Wang, Xiao, Li, Liu, Liu: "Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters." dans: PLoS ONE, Vol. 11, Issue 2, pp. e0149440, (2016) (PubMed).

Miao, Guo, Li, Ma: "Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury." dans: Neural regeneration research, Vol. 11, Issue 2, pp. 278-84, (2016) (PubMed).

Zhang, Song, Jia, Zhang: "Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair." dans: Neural regeneration research, Vol. 11, Issue 3, pp. 435-40, (2016) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." dans: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet:

Synonyms: Hematopoietic progenitor cell antigen CD34,CD34,CD34,

Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Sequence Similarities: Belongs to the CD34 family.

Poids moléculaire: 105 kDa

ID gène: 947

UniProt: P28906