LIF anticorps (AA 23-202)

N° du produit ABIN3043403

L’anticorps Lapin Polyclonal anti-LIF a été validé pour WB et IHC. Il convient pour détecter LIF dans des échantillons de Humain. Il y a 7+ publications disponibles.

Aperçu rapide pour LIF anticorps (AA 23-202) (ABIN3043403)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-LIF anticorps

Épitope: AA 23-202

Fonction: Anti-LIF Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-LIF Antibody Picoband® (ABIN3043403). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Bamberger, A.-M., Jenatschke, S., Schulte, H. M., Loning, T., Bamberger, C. M. Leukemia inhibitory factor (LIF) stimulates the human HLA-G promoter in JEG3 choriocarcinoma cells. J. Clin. Endocr. Metab. 85: 3932-3936, 2000. 2. Sutherland, G. R., Baker, E., Hyland, V. J., Callen, D. F., Stahl, J., Gough, N. M. The gene for human leukemia inhibitory factor (LIF) maps to 22q12. Leukemia 3: 9-13, 1989.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-LIF (ABIN3043403)

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Dong, Sui, Huang, Wang, Hu, Xiong, Wang, Zhang: "MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice." dans: American journal of translational research, Vol. 8, Issue 2, pp. 1155-63, (2016) (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." dans: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, (2012) (PubMed).

Xu, Sun, Li, Wu, Zhang, Feng: "Pinopodes, leukemia inhibitory factor, integrin-?3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss." dans: Fertility and sterility, Vol. 98, Issue 2, pp. 389-95, (2012) (PubMed).

Li, Xu, Chen, Sun: "Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium." dans: European journal of obstetrics, gynecology, and reproductive biology, Vol. 151, Issue 2, pp. 171-5, (2010) (PubMed).

Pei, Yu, Shi, Jia: "The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women." dans: Contraception, Vol. 82, Issue 4, pp. 379-84, (2010) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." dans: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Détails sur LIF

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet:

Synonyms: Leukemia inhibitory factor,LIF,Differentiation-stimulating factor,D factor,Melanoma-derived LPL inhibitor,MLPLI,Emfilermin,LIF,HILDA,

Tissue Specificity: Widely distributed in various tissues.

Background: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Sequence Similarities: Belongs to the LIF/OSM family.

Poids moléculaire: 36 kDa

ID gène: 3976

UniProt: P15018

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding