EIF6 anticorps (AA 66-210)

N° du produit ABIN3043427

L’anticorps Lapin Polyclonal anti-EIF6 a été validé pour WB, IHC et ICC. Il convient pour détecter EIF6 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EIF6 anticorps (AA 66-210) (ABIN3043427)

Antigène: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Détail du produit anti-EIF6 anticorps

Épitope: AA 66-210

Fonction: Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® (ABIN3043427). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur EIF6

Antigène: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Autre désignation: EIF6

Sujet:

Synonyms: Eukaryotic translation initiation factor 6 ,eIF-6 ,B (2)GCN homolog,B4 integrin interactor,CAB,p27 (BBP),EIF6 ,EIF3A, ITGB4BP ,OK/SW-cl.27,

Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Sequence Similarities: Belongs to the WD repeat ESC family.

Poids moléculaire: 26 kDa

ID gène: 3692

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly