EIF6 anticorps (Middle Region)

N° du produit ABIN3044059

Détail du produit anti-EIF6 anticorps

Antigène: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Épitope: AA 82-96, Middle Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Séquence: QHIRNSLPDT VQIRR

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: eukaryotic translation initiation factor 6
Protein Name: Eukaryotic translation initiation factor 6

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human EIF6(82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EIF6

Antigène: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Autre désignation: EIF6 (EIF6 Produits)

Synonymes: anticorps CAB, anticorps EIF3A, anticorps ITGB4BP, anticorps b(2)gcn, anticorps eIF-6, anticorps p27(BBP), anticorps p27BBP, anticorps 1110004P11, anticorps AA408895, anticorps Itgb4bp, anticorps imc-415, anticorps MGC56562, anticorps itgb4bp4, anticorps zgc:56562, anticorps wu:fc28a04, anticorps wu:ft88f05, anticorps itgb4bp, anticorps eukaryotic translation initiation factor 6, anticorps Eukaryotic translation initiation factor 6, anticorps eukaryotic translation initiation factor 6 S homeolog, anticorps translation initiation factor eIF6, anticorps EIF6, anticorps Eif6, anticorps eif6, anticorps eif-6, anticorps eif6.S, anticorps tif6

Sujet: EIF6(Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Synonyms: b(2)gcn antibody|B(2)GCN homolog antibody|B4 integrin interactor antibody|Binding protein of beta-4 integrin antibody|CAB antibody|eIF-6 antibody|EIF3A antibody|EIF6 antibody|Eukaryotic translation initiation factor 3A antibody|Eukaryotic translation initiation factor 6 antibody|IF6_HUMAN antibody|ITGB4BP antibody|OK/SW-cl.27 antibody|p27 beta 4 integrin binding protein antibody|p27(BBP) antibody|p27BBP antibody|RP4-614O4.1 antibody

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly