CD8 alpha anticorps (AA 22-187)

N° du produit ABIN3043499

Détail du produit anti-CD8 alpha anticorps

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Épitope: AA 22-187

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD8 alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.
Gene Name: CD8a molecule
Protein Name: T-cell surface glycoprotein CD8 alpha chain

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD8 alpha is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CD8 alpha (ABIN3043499)

Xu, Chen, Zhao, Huang, Duan, Tong, Zhang, Li, Chang, Chen: "The CD8? gene in duck (Anatidae): cloning, characterization, and expression during viral infection." dans: Molecular biology reports, Vol. 42, Issue 2, pp. 431-9, (2015) (PubMed).

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." dans: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." dans: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8A (CD8A Produits)

Synonymes: anticorps cd8, anticorps LOC100136450, anticorps BB154331, anticorps Ly-2, anticorps Ly-35, anticorps Ly-B, anticorps Lyt-2, anticorps zgc:136643, anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps RHACD8-4, anticorps CD8a molecule, anticorps uncharacterized LOC100125537, anticorps CD8 alpha, anticorps CD8 antigen, alpha chain, anticorps T-cell surface glycoprotein CD8 alpha chain, anticorps Cd8a, anticorps LOC100125537, anticorps LOC100136450, anticorps CD8A, anticorps cd8a, anticorps LOC100126556

Sujet: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Synonyms: alpha polypeptide (p32) antibody|CD 8a antibody|CD8 alpha chain antibody|CD8 antibody|CD8 antigen alpha chain antibody|CD8 antigen alpha polypeptide antibody|CD8 antigen alpha polypeptide (p32) antibody|CD8a antibody|CD8a antigen antibody|CD8a molecule antibody|CD8A_HUMAN antibody|Leu 2 antibody|Leu2 antibody|Leu2 T lymphocyte antigen antibody|Ly3 antibody|LYT3 antibody|MAL antibody|OKT8 T cell antigen antibody|OTTHUMP00000160760 antibody|OTTHUMP00000160764 antibody|OTTHUMP00000203528 antibody|OTTHUMP00000203721 antibody|p32 antibody|T cell antigen Leu2 antibody|T cell co receptor antibody|T cell coreceptor antibody|T cell surface glycoprotein CD8 alpha chain antibody|T lymphocyte differentiation antigen T8/Leu 2 antibody|T lymphocyte differentiation antigen T8/Leu2 antibody|T-cell surface glycoprotein CD8 alpha chain antibody|T-lymphocyte differentiation antigen T8/Leu-2 antibody|T8 T cell antigen antibody|T8/Leu-2 T-lymphocyte differentiation antigen antibody

ID gène: 925

UniProt: P01732

Pathways: TCR Signaling