Annexin V anticorps (AA 2-320)

N° du produit ABIN3043502

L’anticorps Lapin Polyclonal anti-Annexin V a été validé pour WB, IHC, ICC, FACS, IF et IHC (fro). Il convient pour détecter Annexin V dans des échantillons de Humain, Souris et Rat. Il y a 7+ publications disponibles.

Aperçu rapide pour Annexin V anticorps (AA 2-320) (ABIN3043502)

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Annexin V est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Annexin V anticorps

Épitope: AA 2-320

Fonction: Anti-Annexin V/ANXA5 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Annexin V/ANXA5 Antibody Picoband® (ABIN3043502). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat, -
Immunofluorescence, 2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Bogdanova, N., Horst, J., Chlystun, M., Croucher, P. J. P., Nebel, A., Bohring, A., Todorova, A., Schreiber, S., Gerke, V., Krawczak, M., Markoff, A. A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss.Hum. Molec. Genet. 16: 573-578, 2007. 2. Demange, P., Voges, D., Benz, J., Liemann, S., Gottig, P., Berendes, R., Burger, A., Huber, R. Annexin V: the key to understanding ion selectivity and voltage regulation?Trends Biochem. Sci. 19: 272-276, 1994. 3. Sofiadis A, Becker S, Hellman U, Hultin-Rosenberg L, Dinets A, Hulchiy M, Zedenius J, Wallin G, Foukakis T, Höög A, Auer G, Lehtiö J, Larsson C. (April 2012). "Proteomic profiling of follicular and papillary thyroid tumors.". Eur J Endocrinol.. 166 (4): 657-67.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Annexin V (ABIN3043502)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." dans: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Sun, Kang, Yao, Chen, Sun, An, Jiang, Wang, Hu: "Dehydrocostus lactone suppressed the proliferation, migration, and invasion of colorectal carcinoma through the downregulation of eIF4E expression." dans: Anti-cancer drugs, Vol. 26, Issue 6, pp. 641-8, (2015) (PubMed).

Ji, Li, Wei, Kong, Jin, Chen, Li, Deng: "Effect of celastrol on growth inhibition of prostate cancer cells through the regulation of hERG channel in vitro." dans: BioMed research international, Vol. 2015, pp. 308475, (2015) (PubMed).

Xu, Bi, Li, Fang, Zhou, Li, Chi, Wan, Shen: "Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells." dans: Marine drugs, Vol. 13, Issue 4, pp. 1798-818, (2015) (PubMed).

Hu, Dong, Shi, Deng, Li, Lu: "Evaluation of a novel tetra-functional branched poly(ethylene glycol) crosslinker for manufacture of crosslinked, decellularized, porcine aortic valve leaflets." dans: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 2, pp. 322-36, (2014) (PubMed).

Xiong, Ma, Zhang, Shao, Zhang, Zhou: "Apoptosis induced by ?,?-dimethylacrylshikonin is associated with Bcl-2 and NF-?B in human breast carcinoma MCF-7 cells." dans: Oncology letters, Vol. 6, Issue 6, pp. 1789-1793, (2013) (PubMed).

Jin, Liu, Wang, Xu, Yang, Lu, Sun, Hu: "Protective effects of [Gly14]-Humanin on beta-amyloid-induced PC12 cell death by preventing mitochondrial dysfunction." dans: Neurochemistry international, Vol. 56, Issue 3, pp. 417-23, (2010) (PubMed).

Détails sur Annexin V

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: ANXA5

Sujet:

Synonyms: Annexin A5,Anchorin CII,Annexin V,Annexin-5,Calphobindin I,CBP-I,Endonexin II,Lipocortin V,Placental anticoagulant protein 4,PP4,Placental anticoagulant protein I,PAP-I,Thromboplastin inhibitor,Vascular anticoagulant-alpha,VAC-alpha,ANXA5,ANX5, ENX2, PP4,

Tissue Specificity: Expressed in all cell types so far analyzed.

Background: Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Sequence Similarities: Belongs to the annexin family.

Poids moléculaire: 36 kDa

ID gène: 308

UniProt: P08758

Pathways: Apoptose