AFT1 anticorps (AA 1-271)

N° du produit ABIN3043723

Cet anticorps Lapin Polyclonal détecte spécifiquement AFT1 dans WB, IHC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour AFT1 anticorps (AA 1-271) (ABIN3043723)

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AFT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-AFT1 anticorps

Épitope: AA 1-271

Fonction: Anti-ATF1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-ATF1 Antibody Picoband® (ABIN3043723). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with HumanThe brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. ucman, J., Delattre, O., Desmaze, C., Epstein, A. L., Stenman, G., Speleman, F., Fletchers, C. D. M., Aurias, A., Thomas, G. EWS and ATF-1 gene fusion induced by t(12,22) translocation in malignant melanoma of soft parts. Nature Genet. 4: 341-345, 1993. 2. "Entrez Gene: ATF1 activating transcription factor 1"

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur AFT1

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Autre désignation: ATF1

Sujet:

Synonyms: Cyclic AMP-dependent transcription factor ATF-1,cAMP-dependent transcription factor ATF-1,Activating transcription factor 1,Protein TREB36,ATF1,

Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .

Background: ATF1, also known as activating transcription factor 1, is a protein that in humans is encoded by the ATF1 gene. It is mapped to 12q13.12. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Sequence Similarities: Belongs to the bZIP family. ATF subfamily.

Poids moléculaire: 38 kDa

ID gène: 466

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades