ROCK2 anticorps (AA 652-923)

N° du produit ABIN3043921

Cet anticorps Lapin Polyclonal détecte spécifiquement ROCK2 dans WB et IHC. Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 4+ publications.

Aperçu rapide pour ROCK2 anticorps (AA 652-923) (ABIN3043921)

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ROCK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ROCK2 anticorps

Épitope: AA 652-923

Fonction: Anti-ROCK2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ROCK2 Antibody Picoband® (ABIN3043921). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Takahashi, N., Tuiki, H., Saya, H., Kaibuchi, K. : Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2. Thumkeo, D., Keel, J., Ishizaki, T., Hirose, M., Nonomura, K., Oshima, H., Oshima, M., Taketo, M. M., Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-ROCK2 (ABIN3043921)

Qiao, Zou, Liu, Zhu, Wang, Yang, Jiang, Chen: "ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension." dans: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2567-2572, (2016) (PubMed).

Yan, Wang, Jiang, Chen, He, Mang, Shao, Xu: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." dans: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).

Wang, Wang, Liu, Bai, Zhang, Sun, Wang: "Fluoxetine inhibits monocrotaline-induced pulmonary arterial remodeling involved in inhibition of RhoA-Rho kinase and Akt signalling pathways in rats." dans: Canadian journal of physiology and pharmacology, Vol. 90, Issue 11, pp. 1506-15, (2012) (PubMed).

Ma, Zhang, Li, Liu, Ma, Li, Falck, Manthati, Reddy, Medhora, Jacobs, Zhu: "8,9-Epoxyeicosatrienoic acid analog protects pulmonary artery smooth muscle cells from apoptosis via ROCK pathway." dans: Experimental cell research, Vol. 316, Issue 14, pp. 2340-53, (2010) (PubMed).

Détails sur ROCK2

Antigène: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Autre désignation: ROCK2

Sujet:

Synonyms: Rho-associated protein kinase 2,2.7.11.1,Rho kinase 2,Rho-associated, coiled-coil-containing protein kinase 2,Rho-associated, coiled-coil-containing protein kinase II,ROCK-II,p164 ROCK-2,ROCK2,KIAA0619,

Tissue Specificity: Placenta and lung.

Background: Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Poids moléculaire: 161 kDa

ID gène: 9475

UniProt: O75116

Pathways: Dynamique des Microtubules, Signalisation WNT, Tube Formation