TFAM anticorps (N-Term)

N° du produit ABIN3043945

Détail du produit anti-TFAM anticorps

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Épitope: AA 214-241, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TFAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: EMKSWEEQMI EVGRKDLLRR TIKKQRKY

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: transcription factor A, mitochondrial
Protein Name: Transcription factor A, mitochondrial

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for mtTFA is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-TFAM (ABIN3043945)

Wu, Zhao, Xiao, Peng, Chen, He: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." dans: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).

Mo, Peng, Wang, Peng, Lan, Yu: "Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer." dans: Oncology letters, Vol. 6, Issue 2, pp. 617-623, (2013) (PubMed).

Détails sur TFAM

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Autre désignation: TFAM (TFAM Produits)

Synonymes: anticorps tcf6, anticorps mttf1, anticorps mttfa, anticorps tcf6l1, anticorps tcf6l2, anticorps tcf6l3, anticorps mttfa-A, anticorps xl-mtTFA, anticorps MGC153358, anticorps zgc:153358, anticorps MTTF1, anticorps MTTFA, anticorps TCF6, anticorps TCF6L1, anticorps TCF6L2, anticorps TCF6L3, anticorps AI661103, anticorps Hmgts, anticorps mtTFA, anticorps tsHMG, anticorps Mttfa, anticorps transcription factor A, mitochondrial, anticorps transcription factor A, mitochondrial L homeolog, anticorps TFAM, anticorps tfam.L, anticorps tfam, anticorps Tfam

Sujet: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Synonyms: anscription factor 6-like 1 antibody|Mitochondrial transcription factor 1 antibody| mitochondrial transcription factor A antibody|MtTF1 antibody|mtTFA antibody|TCF 6 antibody| TCF-6 antibody|TCF6 antibody|TCF6L1 antibody|TCF6L2 antibody|TCF6L3 antibody|TFAM antibody| TFAM_HUMAN antibody|Transcription factor 6 antibody|Transcription factor 6 like 2 (mitochondrial transcription factor) antibody|Transcription factor 6 like 2 antibody| Transcription factor 6-like 2 antibody|transcription factor 6-like 3 antibody|Transcription factor A, mitochondrial antibody|Transcription factor A, mitochondrial precursor antibody

ID gène: 7019

UniProt: Q00059

Pathways: Chromatin Binding