TLR7 anticorps (C-Term)

N° du produit ABIN3043948

Cet anticorps Lapin Polyclonal détecte spécifiquement TLR7 dans WB, IHC, FACS et ICC. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour TLR7 anticorps (C-Term) (ABIN3043948)

Antigène: TLR7 (Toll-Like Receptor 7 (TLR7))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TLR7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-TLR7 anticorps

Épitope: AA 1020-1047, C-Term

Fonction: Anti-TLR7 Antibody Picoband®

Séquence: QAHPYFWQCL KNALATDNHV AYSQVFKE

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-TLR7 Antibody Picoband® (ABIN3043948). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7, different from the related mouse sequence by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., Reis e Sousa, C.Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA.Science 303: 1529-1531, 2004. 2. Hayashi, T., Gray, C. S., Chan, M., Tawatao, R., Ronacher, L., McGargill, M. A., Datta, S. K., Carson, D. A., Corr, M.Prevention of autoimmune disease by induction of tolerance to Toll-like receptor 7. 3. Hemmi, H., Kaisho, T., Takeuchi, O., Sato, S., Sanjo, H., Hoshino, K., Horiuchi, T., Tomizawa, H., Takeda, K., Akira, S.Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway.Nature Immun. 3: 196-200, 2002.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-TLR7 (ABIN3043948)

Esancy, Condon, Feng, Kimball, Curtright, Dhaka: "A zebrafish and mouse model for selective pruritus via direct activation of TRPA1." dans: eLife, Vol. 7, (2018) (PubMed).

Détails sur TLR7

Antigène: TLR7 (Toll-Like Receptor 7 (TLR7))

Autre désignation: TLR7

Sujet:

Synonyms: Toll-like receptor 7,TLR7,UNQ248/PRO285,

Tissue Specificity: Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

Background: TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.

Sequence Similarities: Belongs to the Toll-like receptor family.

Poids moléculaire: 121 kDa

ID gène: 51284

Pathways: Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades