Gelsolin anticorps (C-Term)
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- Antigène Voir toutes Gelsolin (GSN) Anticorps
- Gelsolin (GSN)
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Épitope
- AA 763-775, C-Term
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Gelsolin est non-conjugé
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Séquence
- WDDDYWSVDP LDR
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
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Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: gelsolin
Protein Name: Gelsolin - Purification
- Immunogen affinity purified.
- Immunogène
- A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin(763-775aa WDDDYWSVDPLDR), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product GSN Anticorps primaire
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- Indications d'application
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for GSN is approximately 1.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Agent conservateur
- Thimerosal (Merthiolate), Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Date de péremption
- 12 months
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Proteomics of acute heart failure in a rat post-myocardial infarction model." dans: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).
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Proteomics of acute heart failure in a rat post-myocardial infarction model." dans: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).
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- Antigène
- Gelsolin (GSN)
- Autre désignation
- GSN (GSN Produits)
- Synonymes
- anticorps ADF, anticorps AGEL, anticorps CG1106, anticorps DGS, anticorps Dmel\\CG1106, anticorps gel, anticorps scin, anticorps cb107, anticorps gsn, anticorps sb:cb107, anticorps u-gelsolin, anticorps wu:fi16f06, anticorps gelsolin, anticorps Gelsolin, anticorps gelsolin S homeolog, anticorps gelsolin a, anticorps GSN, anticorps Gel, anticorps Gsn, anticorps gsn.S, anticorps gsn, anticorps gsna
- Sujet
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Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor, it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.
Synonyms: Actin depolymerizing factor antibody|Actin-depolymerizing factor antibody|ADF antibody|AGEL antibody|Amyloidosis Finnish type antibody|Brevin antibody|DKFZp313L0718 antibody|GELS_HUMAN antibody|Gelsolin antibody|GSN antibody - UniProt
- P06396
- Pathways
- Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy
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