EIF2S1 anticorps (Middle Region)

N° du produit ABIN3044058

Détail du produit anti-EIF2S1 anticorps

Antigène: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Épitope: AA 123-137, Middle Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2S1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.

Séquence: KDEQLESLFQ RTAWV

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.
Gene Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa
Protein Name: Eukaryotic translation initiation factor 2 subunit 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV).

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for EIF2S1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EIF2S1

Antigène: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Autre désignation: EIF2S1 (EIF2S1 Produits)

Sujet: EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Synonyms: EIF 2 alpha antibody|EIF 2 antibody|EIF 2A antibody|EIF 2alpha antibody|eIF-2-alpha antibody|eIF-2A antibody|EIF-2alpha antibody|EIF2 alpha antibody|EIF2 antibody|EIF2A antibody|EIF2S1 antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha antibody|Eukaryotic translation initiation factor 2 subunit 1 antibody|Eukaryotic translation initiation factor 2 subunit alpha antibody|IF2A_HUMAN antibody

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C