DLL3 anticorps (C-Term)

N° du produit ABIN3044121

Cet anticorps anti-DLL3 est un anticorps Lapin Polyclonal détectant DLL3 dans WB. Adapté pour Humain.

Aperçu rapide pour DLL3 anticorps (C-Term) (ABIN3044121)

Antigène: DLL3 (delta Like Protein 3 (DLL3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DLL3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-DLL3 anticorps

Épitope: AA 599-618, C-Term

Fonction: Anti-Delta-like protein 3 DLL3 Antibody Picoband®

Séquence: RAGQRQHLLF PYPSSILSVK

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Delta-like protein 3 DLL3 Antibody (ABIN3044121). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
1. Bulman, M. P., Kusumi, K., Frayling, T. M., McKeown, C., Garrett, C., Lander, E. S., Krumlauf, R., Hattersley, A. T., Ellard, S., Turnpenny, P. D. Mutations in the human delta homologue,DLL3, cause axial skeletal defects in spondylocostal dysostosis.Nature Genet. 24: 438-441, 2000. 2. Dunwoodie, S. L., Henrique, D., Harrison, S. M., Beddington, R. S. P. Mouse Dll3: a novel divergent delta gene which may complement the function of other delta homologues during early pattern formation in the mouse embryo. Development 124: 3065-3076, 1997. 3. Kusumi, K., Sun, E. S., Kerrebrock, A. W., Bronson, R. T., Chi, D.-C., Bulotsky, M. S., Spencer, J. B., Birren, B. W., Frankel, W. N., Lander, E. S. The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries.Nature Genet. 19: 274-278, 1998.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur DLL3

Antigène: DLL3 (delta Like Protein 3 (DLL3))

Autre désignation: DLL3

Sujet:

Synonyms: Delta-like protein 3,Drosophila Delta homolog 3,Delta3,DLL3,

Tissue Specificity: Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells. .

Background: DLL3 (DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene (Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908 (Bulman et al., 2000). Dunwoodie et al. (1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.

Sequence Similarities: Contains 1 DSL domain.

Poids moléculaire: 65 kDa

Pathways: Signalisation Notch