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Hexosaminidase A anticorps (Middle Region)

HEXA Reactivité: Humain, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN3044173
  • Antigène Voir toutes Hexosaminidase A (HEXA) Anticorps
    Hexosaminidase A (HEXA)
    Épitope
    • 8
    • 8
    • 8
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 191-207, Middle Region
    Reactivité
    • 60
    • 25
    • 13
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Rat
    Hôte
    • 51
    • 13
    Lapin
    Clonalité
    • 53
    • 11
    Polyclonal
    Conjugué
    • 38
    • 5
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Hexosaminidase A est non-conjugé
    Application
    • 51
    • 34
    • 13
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Fonction
    Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha(HEXA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Séquence
    DVMAYNKLNV FHWHLVD
    Réactivité croisée (Details)
    Predicted Cross Reactivity: mouse
    No cross reactivity with other proteins.
    Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
    Attributs du produit
    Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha(HEXA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: hexosaminidase A(alpha polypeptide)
    Protein Name: Beta-hexosaminidase subunit alpha
    Purification
    Immunogen affinity purified.
    Immunogène
    A synthetic peptide corresponding to a sequence in the middle region of human HEXA(191-207aa DVMAYNKLNVFHWHLVD), different from the related rat and mouse sequences by one amino acid.
    Isotype
    IgG
    Top Product
    Discover our top product HEXA Anticorps primaire
  • Indications d'application
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Commentaires

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Agent conservateur
    Thimerosal (Merthiolate), Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Date de péremption
    12 months
  • Antigène
    Hexosaminidase A (HEXA)
    Autre désignation
    HEXA (HEXA Produits)
    Synonymes
    anticorps TSD, anticorps fc04h11, anticorps wu:fc04h11, anticorps wu:fv09f06, anticorps si:dkey-35i22.2, anticorps HEXA, anticorps Hex-1, anticorps zgc:112084, anticorps hexosaminidase subunit alpha, anticorps hexosaminidase B (beta polypeptide), anticorps CUGBP Elav-like family member 6, anticorps hexosaminidase A, anticorps hexosaminidase A (alpha polypeptide), anticorps beta-hexosaminidase subunit alpha-like, anticorps HEXA, anticorps hexb, anticorps CELF6, anticorps Hexa, anticorps hexa, anticorps LOC100228061
    Sujet
    HEXA(hexosaminidase A(alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit(amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

    Synonyms: Beta hexosaminidase alpha chain precursor antibody|Beta hexosaminidase subunit alpha antibody|Beta N acetylhexosaminidase antibody|Beta N acetylhexosaminidase subunit alpha antibody|Beta-hexosaminidase subunit alpha antibody|Beta-N-acetylhexosaminidase subunit alpha antibody|Hexa antibody|HEXA_HUMAN antibody|Hexosaminidase A(alpha polypeptide) antibody|Hexosaminidase A alpha polypeptide antibody|Hexosaminidase A antibody|Hexosaminidase subunit A antibody|MGC99608 antibody|N acetyl beta glucosaminidase antibody|N acetyl beta glucosaminidase subunit alpha antibody|N-acetyl-beta-glucosaminidase subunit alpha antibody|TSD antibody
    UniProt
    P06865
    Pathways
    Sensory Perception of Sound, Glycosaminoglycan Metabolic Process
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