HDAC3 anticorps (C-Term)

N° du produit ABIN3044210

L’anticorps Lapin Polyclonal anti-HDAC3 a été validé pour WB, IHC, IF, FACS, ICC et IHC (fro). Il convient pour détecter HDAC3 dans des échantillons de Humain, Souris et Rat. Il y a 1 publication disponible.

Aperçu rapide pour HDAC3 anticorps (C-Term) (ABIN3044210)

Antigène: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-HDAC3 anticorps

Épitope: AA 411-428, C-Term

Fonction: Anti-Histone deacetylase 3 HDAC3 Antibody Picoband®

Séquence: NEFYDGDHDN DKESDVEI

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Histone deacetylase 3 HDAC3 Antibody (ABIN3044210). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC3, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Alenghat, T., Meyers, K., Mullican, S. E., Leitner, K., Adeniji-Adele, A., Avila, J., Bucan, M., Ahima, R. S., Kaestner, K. H., Lazar, M. A. Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology. Nature 456: 997-1000, 2008. 2. Chen, L., Fischle, W., Verdin, E., Greene, W. C. Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001. 3. Dangond F, Hafler DA, Tong JK, Randall J, Kojima R, Utku N, Gullans SR (Mar 1998). "Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells". Biochem Biophys Res Commun 242 (3): 648-52. doi:10.1006/bbrc.1997.8033.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-HDAC3 (ABIN3044210)

Zhao, Chen, Li, Zhang, Li, Das, Bean, Kong, Zhu, Gao: "Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation." dans: BMC neuroscience, Vol. 10, pp. 113, (2009) (PubMed).

Détails sur HDAC3

Antigène: HDAC3 (Histone Deacetylase 3 (HDAC3))

Autre désignation: HDAC3

Sujet:

Synonyms: Histone deacetylase 3,HD3,3.5.1.98,RPD3-2,SMAP45,HDAC3,

Tissue Specificity: Widely expressed.

Background: HDAC3 (HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene. The HDAC3 gene is mapped to 5q31.3. HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53 % amino acid identity with HDAC1 and 52 % with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4. HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.

Sequence Similarities: Belongs to the histone deacetylase family. HD type 1 subfamily.

Poids moléculaire: 45 kDa

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development