CAPNL1 anticorps (Middle Region)

N° du produit ABIN3044292

Cet anticorps anti-CAPNL1 est un anticorps Lapin Polyclonal détectant CAPNL1 dans WB, IHC, ICC, IF et IHC (fro). Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour CAPNL1 anticorps (Middle Region) (ABIN3044292)

Antigène: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CAPNL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CAPNL1 anticorps

Épitope: AA 312-326, Middle Region

Fonction: Anti-Calpain 1/CAPN1 Antibody Picoband®

Séquence: EWNNVDPYER DQLRV

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Calpain 1/CAPN1 Antibody (ABIN3044292). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1, different from the mouse sequence by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence".

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-CAPNL1 (ABIN3044292)

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." dans: Medicine, Vol. 93, Issue 23, pp. e138, (2014) (PubMed).

Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." dans: Microvascular research, Vol. 81, Issue 2, pp. 160-8, (2011) (PubMed).

Détails sur CAPNL1

Antigène: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Autre désignation: CAPN1

Sujet:

Synonyms: Calpain-1 catalytic subunit,3.4.22.52,Calcium-activated neutral proteinase 1,CANP 1,Calpain mu-type,Calpain-1 large subunit,Cell proliferation-inducing gene 30 protein,Micromolar-calpain,muCANP,CAPN1,CANPL1,PIG30,

Tissue Specificity: Ubiquitous.

Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.

Sequence Similarities: Belongs to the peptidase C2 family.

Poids moléculaire: 82 kDa

UniProt: P07384

Pathways: Apoptose