APOA1 anticorps (AA 25-259)

N° du produit ABIN3044502

Cet anticorps anti-APOA1 est un anticorps Lapin Polyclonal détectant APOA1 dans WB et IHC. Adapté pour Rat.

Aperçu rapide pour APOA1 anticorps (AA 25-259) (ABIN3044502)

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APOA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-APOA1 anticorps

Épitope: AA 25-259

Fonction: Anti-Apolipoprotein A I/APOA1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Apolipoprotein A I/APOA1 Antibody Picoband® (ABIN3044502). Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived rat APOA1 recombinant protein (Position: D25-A259). Rat APOA1 shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse APOA1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat
Western blot, 0.1-0.5 μg/mL, Rat
1. Brewer, H. B., Jr., Fairwell, T., LaRue, A., Ronan, R., Houser, A., Bronzert, T. J. : The amino acid sequence of human apoA-I, an apolipoprotein isolated from high absorbance lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004). "Role of apolipoprotein A-I in protecting against endotoxin toxicity". Acta Biochim. Biophys. Sin. (Shanghai) 36 (6): 419-24. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988). "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I". J. Clin. Invest. 82 (3): 803-7. 4. Zhang, Y., Zanotti, I., Reilly, M. P., Glick, J. M., Rothblat, G. H., Rader, D. J. : Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur APOA1

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Autre désignation: Apoa1

Sujet:

Synonyms: Apolipoprotein A-I,Apo-AI,ApoA-I,Apolipoprotein A1,Proapolipoprotein A-I,ProapoA-I,Apoa1,

Tissue Specificity: Major protein of plasma HDL, also found in chylomicrons.

Background: Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Poids moléculaire: 24 kDa

ID gène: 25081

UniProt: P04639

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism