DIABLO anticorps (AA 56-239)

N° du produit ABIN3044515

Cet anticorps Lapin Polyclonal détecte spécifiquement DIABLO dans WB, IHC, ELISA, IF, FACS, IP et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

Aperçu rapide pour DIABLO anticorps (AA 56-239) (ABIN3044515)

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DIABLO est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-DIABLO anticorps

Épitope: AA 56-239

Fonction: Anti-Smac/Diablo Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: coli-derived human Smac,Diablo recombinant protein (Position: A56-D239). Human Smac,Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac,Diablo.

Attributs du produit: Anti-Smac/Diablo Antibody. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-DIABLO (ABIN3044515)

You, Li, Jin, Zheng, Chen, Yang: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." dans: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).

Détails sur DIABLO

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Autre désignation: DIABLO

Sujet:

Background: Diablo homolog  (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO  ( IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis.  A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: diablo IAP-binding mitochondrial protein

Poids moléculaire: 20 kDa

ID gène: 56616

UniProt: Q9NR28

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity