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His Tag anticorps (FITC)

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans IHC et FACS. Il présente une réactivité envers .
N° du produit ABIN304882

Aperçu rapide pour His Tag anticorps (FITC) (ABIN304882)

Antigène

Voir toutes His Tag Anticorps
His Tag

Reactivité

Veuillez nous consulter SVP

Hôte

  • 110
  • 66
  • 11
  • 10
  • 9
  • 8
  • 2
  • 1
Souris

Clonalité

  • 125
  • 77
  • 11
Monoclonal

Conjugué

  • 97
  • 22
  • 14
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  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
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  • 1
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Cet anticorp His Tag est conjugé à/à la FITC

Application

  • 172
  • 120
  • 41
  • 32
  • 32
  • 30
  • 28
  • 16
  • 15
  • 13
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  • 13
  • 7
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Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone

AD1-1-10
  • Séquence

    HHHHHH

    Specificité

    Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

    Purification

    Protein G purified

    Immunogène

    PAX6 transcription factor linked to histidine tag.

    Type of Immunogen: Fusion protein

    Isotype

    IgG1
  • Indications d'application

    Approved: Flo (1:10), IHC

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 0.09 % sodium azide, 1 % BSA.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
  • Antigène

    His Tag

    Classe de substances

    Tag
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