LEF1 anticorps (full length)

N° du produit ABIN306681

Cet anticorps anti-LEF1 Monoclonal Souris (Clone REMB6) (ABIN306681) détecte spécifiquement LEF1 dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour LEF1 anticorps (full length) (ABIN306681)

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: REMB6

Détail du produit anti-LEF1 anticorps

Épitope: full length

Fonction: Mouse anti Human LEF-1

Attributs du produit: The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB6 recognizes the High Mobility Group DNA domain of LEF-1

Purification: Protein A/G Chromatography

Immunogène: Full-length recombinant LEF-1 protein

Isotype: IgG1

Information d'application

Indications d'application: Antibody can be used for Western blotting (1-5 μg/mL) and immunofluorescence on paraformaldehyde-fixed cells (1:1000 or 1 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF/TCF/HMG

Sujet: Lef-1, mouse anti human lymphoid enhancer factor-1, T-cell specific transcription factor 1-alpha, TCF-1alpha

UniProt: Q9UJU2

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding