CAPN1 anticorps

N° du produit ABIN306692

L’anticorps Souris Monoclonal anti-CAPN1 a été validé pour WB, IP et EIA. Il convient pour détecter CAPN1 dans des échantillons de Humain.

Aperçu rapide pour CAPN1 anticorps (ABIN306692)

Antigène: CAPN1 (Calpain 1 (CAPN1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CAPN1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 28F3

Détail du produit anti-CAPN1 anticorps

Réactivité croisée: Boeuf (Vache), Humain

Attributs du produit: Mouse anti calpain I, II small subunit, p30,The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and μcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Isotype: IgG1

Information d'application

Indications d'application: Use at 0.5-1 μg/mL for ELISA and western blot, 1-2 μg/mL for immunoprecipitation.

Restrictions: For Research Use only

Stockage

Buffer: Provided as a concentration of 1 mg/mL in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol, 3 mM sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur CAPN1

Antigène: CAPN1 (Calpain 1 (CAPN1))

Autre désignation: Calpain I

UniProt: P17655