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CAPN1 anticorps (AA 625-713)

Name: CAPN1 anticorps (AA 625-713)
SKU: ABIN737646
Price: 414.62 EUR
Availability: InStock

CAPN1 Reactivité: Humain, Souris, Rat WB, IP, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse liver tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

ELISA

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

4 images

(2 références)

N° du produit ABIN737646

Fiche de données

Antigène Voir toutes CAPN1 Anticorps

CAPN1 (Calpain 1 (CAPN1))
Épitope
15

6

5

3

2

2

1

1

1

1

1

1

1
AA 625-713
Reactivité
44

31

31

4

2

2

2

2

1

1

1

1

1

1

1

1
Humain, Souris, Rat
Hôte
52

5

1
Lapin
Anticorps secondaires appropriés
Clonalité
54

4
Polyclonal
Conjugué
22

10

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CAPN1 est non-conjugé
Application
49

18

17

14

12

12

11

9

7

6

4

2

2

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée

Humain, Souris, Rat

Homologie

Cow,Sheep,Pig,Chicken

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from mouse Calpain 1

Isotype

IgG

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anti-Calpain 1 (CAPN1) (AA 75-356) antibody
CAPN1 Reactivité: Rat WB, IHC, ICC Hôte: Lapin Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 185-234) antibody
CAPN1 Reactivité: Humain, Boeuf (Vache), Porc, Chévre, Lapin, Mouton, Roussette (Chauve-souris), Poulet, Chien, Cobaye, Cheval, Singe, Xenopus laevis, Poisson zèbre (Danio rerio) WB Hôte: Lapin Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 300-349) antibody
CAPN1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chévre, Lapin, Mouton WB Hôte: Lapin Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 515-526) antibody
CAPN1 Reactivité: Humain WB, IHC, ELISA, IHC (p) Hôte: Chèvre Polyclonal unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Li, Chen, Lu, Zhang, Guan, Li, Wang: "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model." dans: International journal of molecular sciences, Vol. 18, Issue 8, (2017) (PubMed).

Miners, Renfrew, Swirski, Love: "Accumulation of ¿-synuclein in dementia with Lewy bodies is associated with decline in the ¿-synuclein-degrading enzymes kallikrein-6 and calpain-1." dans: Acta neuropathologica communications, Vol. 2, Issue 1, pp. 164, (2014) (PubMed).
Antigène

CAPN1 (Calpain 1 (CAPN1))

Autre désignation

Calpain 1 (CAPN1 Produits)

Synonymes

anticorps CAPN1, anticorps Capa-1, anticorps Capa1, anticorps mu-calpin, anticorps Capn1, anticorps fj67g01, anticorps wu:fj67g01, anticorps calpain 1, anticorps zgc:55262, anticorps CAPN1, anticorps Capn1, anticorps zgc:55262

Sujet

Synonyms: Capa1, Capa-1, mu-calpin, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain, muCANP, Capn1, Canp1
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

ID gène

12333

UniProt

O35350