LEF1 anticorps (all Isoforms)

N° du produit ABIN306725

L’anticorps Souris Monoclonal anti-LEF1 a été validé pour WB. Il convient pour détecter LEF1 dans des échantillons de Humain.

Aperçu rapide pour LEF1 anticorps (all Isoforms) (ABIN306725)

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB)

Clone: 1C3-1D10

Détail du produit anti-LEF1 anticorps

Épitope: all Isoforms

Réactivité croisée: Humain

Attributs du produit: Anti-Lymphoid Enhancer Factor-1 (All Isoforms) Monoclonal Antibody,Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.

Purification: Protein G Chromatography

Immunogène: Mice were immunized with a recombinant full length LEF-1 protein

Isotype: IgG1

Information d'application

Indications d'application: Antibody detects all LEF-1 isoforms by Western blot. Does not cross-react with TCF-1 or TCF-4. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF-1

UniProt: Q9UJU2

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding