RIT1 anticorps

N° du produit ABIN306804

Cet anticorps anti-RIT1 est un anticorps Souris Monoclonal détectant RIT1 dans WB. Adapté pour Humain.

Aperçu rapide pour RIT1 anticorps (ABIN306804)

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RIT1 est non-conjugé

Application: Western Blotting (WB)

Clone: 14G7

Détail du produit anti-RIT1 anticorps

Réactivité croisée: Humain, Souris

Attributs du produit: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Purification: Protein A or G purified

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.

Isotype: IgG1

Information d'application

Indications d'application: Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 μg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol at pH 7.5 and 3 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur RIT1

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Autre désignation: Rit

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway