BrdU anticorps (FITC)

N° du produit ABIN306900

Cet anticorps anti-BrdU est un anticorps Souris Monoclonal détectant BrdU dans IHC et FACS. Adapté pour .

Aperçu rapide pour BrdU anticorps (FITC) (ABIN306900)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est conjugé à/à la FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: MoBU-1

Détail du produit anti-BrdU anticorps (FITC)

Attributs du produit: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.

Purification: Protein A/G Chromatography

Immunogène: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin was used to immunize BALB/c mice

Isotype: IgG1

Information d'application

Indications d'application: Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern. Use at 1-5 μg/106 cells for flow cytometry. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU (bromodeoxyuridine)

Classe de substances: Chemical