MYOD1 anticorps

N° du produit ABIN307158

Cet anticorps anti-MYOD1 Monoclonal Souris (Clone 5-8A) (ABIN307158) détecte spécifiquement MYOD1 dans ELISA, IF, IP, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Poulet.

Aperçu rapide pour MYOD1 anticorps (ABIN307158)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5-8A

Détail du produit anti-MYOD1 anticorps

Fonction: Mouse anti Human MyoD1

Réactivité croisée: Poulet, Humain, Souris, Rat

Attributs du produit: Recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilm79s tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing79s sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm79s tumors.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Isotype: IgG1

Information d'application

Indications d'application: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate),and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD1

Sujet: Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3

Poids moléculaire: 45kDa

UniProt: P15172, P10085, Q02346, P16075

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development