Hexokinase 1 anticorps (N-Term)

N° du produit ABIN317095

Cet anticorps Souris Monoclonal détecte spécifiquement Hexokinase 1 dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour Hexokinase 1 anticorps (N-Term) (ABIN317095)

Antigène: Hexokinase 1 (HK1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Hexokinase 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 4D7

Détail du produit anti-Hexokinase 1 anticorps

Épitope: AA 1-917, N-Term

Specificité: The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse (see pictures below).

Attributs du produit: Synonyms: HK1, Hexokinase type I, Brain form hexokinase

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: Recombinant Human Hexokinase 1 (1-917 aa) purified from E. coli

Isotype: IgG2a

Information d'application

Indications d'application: ELISA. Western blot (1: 1,000). Immunofluorescence (1: 1,000). Immunohistochemistry on Paraffin Sections (5 μg/mL): This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Hexokinase 1

Antigène: Hexokinase 1 (HK1)

Autre désignation: Hexokinase-1

Sujet: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.Synonyms: Brain form hexokinase, HK1, Hexokinase type I

ID gène: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, L'effet Warburg