APP anticorps (AA 737-751)

N° du produit ABIN319013

L’anticorps anti-APP Polyclonal Lapin est utilisé pour la détection de APP dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour APP anticorps (AA 737-751) (ABIN319013)

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-APP anticorps

Épitope: AA 737-751

Specificité: This antibody recognises both intact APP, and also the C99 fragment generated by Beta-secretase. The sequence recognised by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide which is found in the brains of Alzheimer's disease patients.

Purification: Affinity Chromatography

Immunogène: Synthetic peptide corresponding to amino acids 737-751 of Human APP

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, 0.02 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur APP

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Autre désignation: Amyloid beta A4 Protein / APP

Sujet: Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

ID gène: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour