Dynamin 1 anticorps (pSer774)

N° du produit ABIN319098

Cet anticorps anti-Dynamin 1 est un anticorps Mouton Polyclonal détectant Dynamin 1 dans WB, IF et IHC (fro). Adapté pour Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet et Singe.

Aperçu rapide pour Dynamin 1 anticorps (pSer774) (ABIN319098)

Antigène: Dynamin 1 (DNM1)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Singe

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Dynamin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Dynamin 1 anticorps

Épitope: pSer774

Specificité: This antibody is specific for Dynamin when phosphorylated at Serine 774.

Réactivité croisée (Details): Species reactivity (expected):Human, Monkey, Bovine, Chicken, Mouse, Dog.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogène: Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774. Genename: DNM

Isotype: IgG

Information d'application

Indications d'application: Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95 kDa in Rathippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10 mMSodium Fluoride should be added to the sample buffer. Milk or other casein-basedblocking solutions are not recommended as casein is a Phosphoprotein and its use canresult in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Dynamin 1

Antigène: Dynamin 1 (DNM1)

Autre désignation: Dynamin-1

Sujet: Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.Synonyms: DNM, DNM1

ID gène: 140694

NCBI Accession: NP_542420

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling