PC anticorps

N° du produit ABIN3197484

Il existe 2+ publications pour ce produit. L’anticorps anti-PC Polyclonal Lapin est utilisé pour la détection de PC dans des échantillons de Spinach, Zea mays, Synechocystis, Arabidopsis thaliana, Potato, Pea, Oryza sativa, Nicotiana tabacum, Mustard, Marchantia polymorpha, Lathyrus sativus, Helianthus annuus, Chlamydomonas reinhardtii et Orge. Il a été validé pour WB et EM.

Aperçu rapide pour PC anticorps (ABIN3197484)

Antigène: PC

Reactivité: Spinach, Zea mays, Synechocystis, Arabidopsis thaliana, Potato, Pea, Oryza sativa, Nicotiana tabacum, Mustard, Marchantia polymorpha, Lathyrus sativus, Helianthus annuus, Chlamydomonas reinhardtii, Orge

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Electron Microscopy (EM)

Détail du produit

Fonction: Anti-PC | Plastocyanin

Réactivité croisée (Details): Arabidops thaliana, Brassica juncea, Chlamydomonas reinhardtii, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Marchantia polymorpha, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Dicots,Chlamydomonas reinhardtii,Nicotiana benthamiana,Physcomitrium patens,Ricinus communis,Solanum lycopersicum,Suaeda salsa

Purification: Serum

Immunogène: Purified native plastocyanin from Spinacia oleracea UniProt: P00289

Information d'application

Indications d'application: 1 : 100 (IG), 1 : 2000 (WB)

Commentaires:

plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Référence

Perea-García, Garcia-Molina, Andrés-Colás, Vera-Sirera, Pérez-Amador, Puig, Peñarrubia: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." dans: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." dans: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Détail du antigène

Antigène: PC

Sujet:

Background: Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Additional information: Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested.

Poids moléculaire: 10 kDa

ID gène: 838622

UniProt: P00289