AGO4 anticorps

N° du produit ABIN3197495

L’anticorps anti-AGO4 Polyclonal Lapin est utilisé pour la détection de AGO4 dans des échantillons de Arabidopsis thaliana et Hyacinthus orientalis (Common hyacinth). Il a été validé pour WB, IP, ICC et Co-IP.

Aperçu rapide pour AGO4 anticorps (ABIN3197495)

Antigène: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivité: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO4 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Détail du produit anti-AGO4 anticorps

Fonction: Anti-AGO4 | Argonaute 4

Réactivité croisée (Details): Arabidopsis thaliana, Hyacinthus orientalis
Not reactive in: Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Poplar sp.,Solanum lycopersicum, Vigna angularis, Zea mays

Homologie: Arabidopsis thaliana

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5

Information d'application

Indications d'application: 1 : 100 (ICC), 5 μg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.

Détails sur AGO4

Antigène: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Autre désignation: AGO4

Sujet: AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Poids moléculaire: 103 kDa

ID gène: 817246

UniProt: Q9ZVD5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process