AGO4 anticorps

N° du produit ABIN3197495

Détail du produit anti-AGO4 anticorps

Antigène: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivité: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO4 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Réactivité croisée (Details): Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis

Homologie: Arabidopsis thaliana

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 103 kDa
Antibody concentration is 0.4 mg/mL in 50% glycerol, PBS, 0.005% thimerosal,0.05% BSA, threfore the antibody sample will not freeze in -20°C.binds enDog (Canine)enous siRNAs, preference for 24nt siRNAs with 5' A.

Purification: serum

Immunogène: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.

Information d'application

Indications d'application: 1: 4000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Buffer: Antibody concentration is 0.4 mg/mL in 50 % glycerol, PBS, 0.005 % thimerosal,0.05 % BSA. Please, add 125 µl of deionized water and let it stand in 4 °C overnight to mix. Afterwards warm the antibody sample up to RT and allow theglycerol to dissolve well. Mak

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Stock: -20 °C

Stockage commentaire: Store at -20°C, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.

Détails sur AGO4

Antigène: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Autre désignation: AGO4 (EIF2C4 Produits)

Synonymes: anticorps EIF2C4, anticorps ago4, anticorps Argonaute4, anticorps argonaute-4, anticorps eif2c4, anticorps wu:fd14f04, anticorps ARGONAUTE 4, anticorps OCP11, anticorps OVEREXPRESSOR OF CATIONIC PEROXIDASE 11, anticorps T20P8.9, anticorps T20P8_9, anticorps Eif2c4, anticorps 5730550L01Rik, anticorps AI481660, anticorps argonaute 4, RISC catalytic component, anticorps argonaute 1, RISC catalytic component, anticorps argonaute RISC catalytic component 4, anticorps Argonaute family protein, anticorps argonaute 4, RISC catalytic component L homeolog, anticorps argonaute RISC catalytic subunit 4, anticorps AGO4, anticorps ago1, anticorps ago4, anticorps Ago4, anticorps ago4.L

Sujet: AGI Code: At2g27040
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Poids moléculaire: 103 kDa

UniProt: Q9ZVD5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process