Integrin beta 4 anticorps

N° du produit ABIN319862

L’anticorps Souris Monoclonal anti-Integrin beta 4 a été validé pour WB, FACS, IP et IHC (fro). Il convient pour détecter Integrin beta 4 dans des échantillons de Humain.

Aperçu rapide pour Integrin beta 4 anticorps (ABIN319862)

Antigène: Integrin beta 4 (ITGB4)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 450-9D

Détail du produit anti-Integrin beta 4 anticorps

Specificité: This antibody reacts with beta 4 Integrin (CD104). This antibody recognises an extracellular epitope on the CD104 Molecule.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: Purified alpha 6/beta 4 Integrin from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry (1/25-1/100): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Western blot. Immunohistochemistry on Frozen Sections (1/500-1/1000). Does not work on Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Integrin beta 4

Antigène: Integrin beta 4 (ITGB4)

Autre désignation: CD104 / Integrin beta-4

Sujet: Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA), also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. CD104 is expressed on epithelial cells, Schwann cells and some tumor cell lines.Synonyms: GP150, ITGB4

ID gène: 3691

NCBI Accession: NP_000204

UniProt: P16144

Pathways: Integrin Complex