His Tag anticorps

N° du produit ABIN319985

L’anticorps anti-His Tag Monoclonal Souris est utilisé pour la détection de His Tag. Il a été validé pour WB, IP, IHC (fro) et EIA.

Aperçu rapide pour His Tag anticorps (ABIN319985)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AD1-1-10

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Purification: Affinity Chromatography on Protein G

Immunogène: PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Immunhistology on Frozen Sections. ELISA. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as a preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.