CCR7 anticorps

N° du produit ABIN322907

L’anticorps Rat Monoclonal anti-CCR7 a été validé pour FACS, IHC, Func et Neut. Il convient pour détecter CCR7 dans des échantillons de Souris.

Aperçu rapide pour CCR7 anticorps (ABIN322907)

Antigène: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CCR7 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Neutralization (Neut)

Détail du produit anti-CCR7 anticorps

Specificité: Recognizes mouse CCR7.

Purification: Protein G purified

Immunogène: RBC-2H3 cells expressing mCCR7.

Type of Immunogen: Cells

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, Func, IHC (25 μg/mL), Neut

Usage: Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 μg/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 μg/mL in the presence of 25 ng/mL of r  mMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CCR7

Antigène: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Autre désignation: CCR7

Sujet: Name/Gene ID: CCR7
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7

ID gène: 1236

UniProt: P32248

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process