ERCC1 anticorps (N-Term)

N° du produit ABIN334392

Cet anticorps anti-ERCC1 est un anticorps Chèvre Polyclonal détectant ERCC1 dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour ERCC1 anticorps (N-Term) (ABIN334392)

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ERCC1 est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-ERCC1 anticorps

Épitope: N-Term

Fonction: ERCC1

Séquence: DPGKDKEGVP QPS

Specificité: This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1, NP_001974.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ERCC1

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Autre désignation: ERCC1

Sujet: ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein

ID gène: 2067

NCBI Accession: NP_973730, NP_001974

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response