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ITCH anticorps (Internal Region)

Verified ITCH Reactivité: Humain ELISA, WB, IF, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN334426
  • Antigène Voir toutes ITCH Anticorps
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Épitope
    • 10
    • 8
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 39
    • 36
    • 10
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 46
    • 5
    Chèvre
    Clonalité
    • 45
    • 6
    Polyclonal
    Conjugué
    • 29
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ITCH est non-conjugé
    Application
    • 36
    • 32
    • 7
    • 5
    • 3
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
    Fonction
    ITCH / AIF4
    Séquence
    EIKSHDLKPN GGN
     Réactivité croisée
    Boeuf (Vache), Chien, Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
    Isotype
    IgG
  • Indications d'application

    Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
    Autre désignation
    ITCH (ITCH Produits)
    Synonymes
    anticorps AIF4, anticorps AIP4, anticorps NAPP1, anticorps dJ468O1.1, anticorps 6720481N21Rik, anticorps 8030492O04Rik, anticorps A130065M08, anticorps C230047C07Rik, anticorps itchy E3 ubiquitin protein ligase, anticorps itchy, E3 ubiquitin protein ligase, anticorps ITCH, anticorps Itch
    Sujet
    ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui
    ID gène
    83737, 16396, 311567
    NCBI Accession
    NP_113671, NP_001244066, NP_001244067
    Pathways
    Activation of Innate immune Response, CXCR4-mediated Signaling Events
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