MBD2 anticorps (Internal Region)

N° du produit ABIN334432

Cet anticorps Chèvre Polyclonal détecte spécifiquement MBD2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour MBD2 anticorps (Internal Region) (ABIN334432)

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-MBD2 anticorps

Épitope: Internal Region

Fonction: MBD2 (isoform 1)

Séquence: RNDPLNQNKG KPDLN

Specificité: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-MBD2 (ABIN334432)

Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." dans: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." dans: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

Détails sur MBD2

Antigène: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Autre désignation: MBD2

Sujet: MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41

ID gène: 8932, 17191

NCBI Accession: NP_003918

Pathways: Chromatin Binding