PINK1 anticorps (Internal Region)

N° du produit ABIN334460

L’anticorps Chèvre Polyclonal anti-PINK1 a été validé pour WB, ELISA et FACS. Il convient pour détecter PINK1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour PINK1 anticorps (Internal Region) (ABIN334460)

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PINK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-PINK1 anticorps

Épitope: Internal Region

Fonction: PINK1

Séquence: QGKAHLESRS YQEAQ

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QGKAHLESRSYQEAQ, from the internal region of the protein sequence according to NP_115785.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 60-65 kDa band observed in lysates of cell lines Jurkat and HeLa (calculated MW of 62.8 kDa according to NP_115785.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PINK1 (ABIN334460)

Choubey, Cagalinec, Liiv, Safiulina, Hickey, Kuum, Liiv, Anwar, Eskelinen, Kaasik: "BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria." dans: Autophagy, Vol. 10, Issue 6, pp. 1105-19, (2014) (PubMed).

Détails sur PINK1

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Autre désignation: PINK1

Sujet: PINK1, PTEN induced putative kinase 1 , BRPK, FLJ27236, PARK6 , protein kinase BRPK, serine/threonine-protein kinase PINK1

ID gène: 65018, 68943, 298575

NCBI Accession: NP_115785

Pathways: Autophagy