HNF1A anticorps (Internal Region)

N° du produit ABIN334504

Cet anticorps Chèvre Polyclonal détecte spécifiquement HNF1A dans ELISA, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour HNF1A anticorps (Internal Region) (ABIN334504)

Antigène: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HNF1A est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-HNF1A anticorps

Épitope: Internal Region

Fonction: TCF1 / HNF1

Séquence: DELPTKKGRR NRFK

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DELPTKKGRRNRFK, from the internal region of the protein sequence according to NP_000536.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: A customer reported a weak band at approx. 75 kDa on lysate of cell line Caco-2, at a concentration of 3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur HNF1A

Antigène: HNF1A (HNF1 Homeobox A (HNF1A))

Autre désignation: HNF1A

Sujet: TCF1, transcription factor 1, hepatic LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor , HNF1, HNF1A, LFB1, MODY3 , Interferon production regulator factor (HNF1), albumin proximal factor, hepatic nuclear factor 1, maturity onset diabetes of t

ID gène: 6927, 21405, 24817

NCBI Accession: NP_000536

Pathways: Hormone Transport, Carbohydrate Homeostasis