Ascorbate Oxidase anticorps (Biotin)

N° du produit ABIN334591

Cet anticorps Lapin Polyclonal détecte spécifiquement Ascorbate Oxidase dans ELISA et WB. Il présente une réactivité avec des échantillons de Cucurbita pepo.

Aperçu rapide pour Ascorbate Oxidase anticorps (Biotin) (ABIN334591)

Antigène: Ascorbate Oxidase (AO)

Reactivité: Cucurbita pepo

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ascorbate Oxidase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Détail du produit anti-Ascorbate Oxidase anticorps (Biotin)

Fonction: Anti-AO | ascorbate oxidase, Biotin conjugated

Réactivité croisée (Details): Cucurbita sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Glycne max,Oryza sativa

Purification: Purified IgG

Immunogène: ascorbate oxidase purified from Cucurbita sp.

Isotype: IgG

Information d'application

Indications d'application: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Commentaires:

IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 1 mL of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily

Buffer: Purified IgG in PBS pH 7.2.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

Détails sur Ascorbate Oxidase

Antigène: Ascorbate Oxidase (AO)

Sujet:

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody

Poids moléculaire: 61 | 45 kDa (Cucurbita pepo)